Characterization of pulsed light for microbial inactivation

Pulsed light is an emerging technology that can serve as alternative to current antimicrobial interventions in the food industry. The first objective of this study was define spectrum and energy characteristics pulsed light. second establish germicidal response Escherichia coli, Salmonella enterica subsp. ser. Typhimurium, Listeria monocytogenes, Bacillus cereus (vegetative cells endospores), Aspergillus niger spores, Penicillium roqueforti spores were evaluated following treatments. Microorganisms treated using three different broad-spectrum xenon gas flashlamps (Type A: 380–1100 nm, Type B: 235–1100 C: 190–1100 nm) for up 15 pulses. All microorganisms experienced a significant interaction flashlamp type × treatment duration (P < 0.0001). Additionally, E. coli with B filters block ultraviolet selectively deliver visible (VIS; 380–740 nm), near-infrared (NIR; 700–1080 VIS-NIR (400–1080 radiation cells. filter VIS, NIR, treatments achieved modest reductions 0.72, 0.19, 0.83 Log10 CFU/mL, respectively, after 75 consecutive To visualize physical effects on cell structures, transmission electron microscopy (TEM) images obtained pulse types placement. TEM imaging indicated damage morphology by negligible. These results indicate microbial sensitivity differs species predominantly attributed portion spectrum.